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Room temperature time is cumulative

For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C

Simulation system equilibration and energy minimization Following preparation, the systems were energy minimized with an energy tolerance of 100 kj/mol and equilibrated in six sequential steps involving loosening of positional restraints on lipid atoms, protein backbone atoms, and protein side chain atoms (equilibration parameters in Extended Data Table 2)

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