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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions

C.01, Wallingford, CT, 2016) The metal atom was described using the Los Alamos (LANL2) effective core potential with the corresponding triple-zeta basis set while all other atoms were described with the Pople double-zeta basis set with a single set of polarization functions on non-hydrogen atoms (6-31 G(d))

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