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Total protein of the cells was extracted and reduced in the presence of 100 mmol/L DTT for 5 min on the boiling water bath, and subsequently alkylated with 50 mol/L iodoacetamide for 30 min at room temperature in the dark
But limited by the availability of specific inhibitors, most of these studies are performed using genetic interference techniques
Additionally, iron chelating agents, including chloroiodohydroxyquine and its derivatives, as well as antioxidants, have shown efficacy in animal models of AD, though clinical trials are yet to be conducted (27,106,116)
10.1016/S0261-5614(03)00108-0 24 DransfeldO.GehrmannT.KhrerK.KircheisG.HolneicherC.HussingerD.et al (2005)