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The freeze-drying process removes water, halting hydrolysis reactions that cleave peptide bonds

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For Syn 1103 and Syn 1103 -104C constructs, eluted fractions were further purified using a HiTrap Heparin HP column (Cytiva, MA, USA), leveraging cation-exchange conditions due to their higher isoelectric point (pI ~9.5)

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Emma F, Nesterova G, Langman C, Labbe A, Cherqui S, Goodyer P, Janssen MC, Greco M, Topaloglu R, Elenberg E, Dohil R, Trauner D, Antignac C, Cochat P, Kaskel F, Servais A, Wuhl E, Niaudet P, Vant Hoff W, Gahl W, Levtchenko E (2014) Nephropathic cystinosis: an international consensus document