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Hydrogen sulfide (H 2 S), a gaseous signaling molecule produced by some cells within the body, acts as a vasodilator (relaxes blood vessels) and thus may have cardioprotective properties (51, 52)

If you want more detail on timing, How Long Does It Take for Glutathione to Work

A mobile phase of 2.5 mM H 2 SO 4 at a 0.5 mL/min flow rate was used, and the column was operated at 55 C [41]

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The ATP level was determined using the ATP Assay Kit from Beyotime, China, following the manufacturers protocol