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Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H

To use Melanotan-1 for tanning protocol, reconstitute lyophilised powder with bacteriostatic water at a 1:1 ratio (typically 1mg peptide per 1mL water), inject 0.250.5mg subcutaneously once daily for 57 days as a loading phase, then switch to maintenance doses of 0.25mg 23 times weekly

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For in vivo experiments, total RNA was extracted from the rat retina using the miRNeasy mini kit (QIAGEN, Hilden, Germany) and then reverse-transcribed into cDNA using the SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific, Waltham, MA, USA)

However, it would be best to take it at the same time each day to avoid the chances of missing a dose