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LC3-II expression was also detected at 4 h, 24 h and 48 h (with a wash at 24 h followed by the addition of fresh media, to remove CSE in order to minimise reduction of cell viability)

Satir P, Christensen ST

Moreover, e-cigarettes increase eosinophil counts and levels of type 2 inflammation-related cytokines IL-4, IL-5, and IL-13 in asthmatic mice, increasing airway hyperresponsiveness and promoting airway inflammation [51]

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