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Where possible, treatment effect for each Arm pair comparison will be expressed using the Hodges-Lehmann estimator with the 95% confidence intervals and significance levels

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Five hundred microliter of the methylene chloride layer containing the nicotine was then vortexed with 500 l of 50 mM HCl solution and the nicotine of the HCl solution was estimated by HPLC analysis at 254 nm