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More specifically, the reactive oxidative species (ROS) affect microenvironments associated with the fluid surrounding the egg, fallopian tube fluid, and abdominal fluid and have a direct bearing on egg quality (cell membranes, mitochondria & DNA), sperm-egg interaction (fertilization), sperm-mediated egg activation, implantation, and early embryo development

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The sample never cooled to a temperature lower than 1500 K between the pulses