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For ultravioletvisible measurements, one-step in vitro reactions with the standard reaction conditions as defined above were performed in a total volume of 1.2 ml in 2 ml microcentrifuge tubes incubated at 30 C for 15 min at 1,200 rpm using a 3 mm shaking diameter

Tavazzi E, et al

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A 2026 review in the American Journal of Sports Medicine emphasizes that despite popularity, "significant research regarding the safety and efficacy of these therapeutic methods is required before definitive recommendations can be made to patients." Only a single human case series exists for BPC-157 (for knee pain), with significant methodological flaws and no controls