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Key Points: i.The golden leaf award: The award created in 2006 by Tobacco Reporter, an international magazine, is given to a company/institution which has introduced public service programmes and has achieved outstanding performance in 5 categories

After static incubation at 30 C for 24 h, the bacteria were collected and the total bacteria RNA were extracted with EasyPure RNA Purification Kit (TransGen Biotech, Beijing, China) following the manufacturers protocol

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Tooth Decay Smoking encourages the growth of harmful bacteria in the mouth, increasing the risk of cavities and tooth decay