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Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air

The most important elements of honey bee nutrition are carbohydrates, proteins, amino acids, and lipids, all of which have a significant impact on individual and colony health (184, 185)

Namgung, S

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For clearer and longer-lasting results, a full course of 812 injections, usually given once or twice a week, is often recommended