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After gradient dilution (10 2 -10 5 ), the bacterial culture was plated separately on 10.0% starch, 10.0% CMC-Na, and 5.0% casein agars and incubated at 37 for 23 days

These factors create a situation where harmful bacteria can grow and produce acids that damage the enamel, which is the protective outer layer of your teeth

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Since the activity of CYP1A2 varies greatly from individual to individual, literature evidenced that there are different influential factors, such as genetics, coffee consumption, oral contraceptive use and smoking, even if the mechanisms are complex and not completely clear [21]