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Methylene blue (MB, Acros Organics), N-Acetyl-L-cysteine (NAC, Acros Organics), and MitoTEMPO (mTEM, Sigma) were dissolved in PBS and added to the growth medium at a final concentration of 100 nM, 100 M, or 100 nM respectively

We acknowledge OpenEye/Cadence for providing us with an academic license for the software used in these studies

Externally added GSH thus, may activate a secondary redox system of GSH-glutaredoxin and might indirectly suggest the absence of GSH synthesis to supplement such reactions

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It also contains magnesium to help the muscles relax and release tension and improve sleep