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We used a standard procedure of described stainings

Due to the hot and arid conditions in which it is grown, the leaf contains significantly lower levels of nicotine than flue-cured Virginia or burley, and harvesting and curing methods are more pristine: The leaves are picked by hand and sun-dried for about a week, a process that enables the leaves to retain some of the natural sugars, which provide a hint of sweetness and a unique taste

The review focused on tobacco-free NPs containing varying nicotine strengths and flavors

This study supports the need for healthcare professionals to recommend tobacco cessation interventions to help prevent PAD, or manage PAD in patients who already have the disease, regarding of whether they are smoking, or consuming tobacco in another form.

doi: 10.1111/j.1600-0714.2008.00669.x