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The mobile phase was consisted of 12% acetonitrile in 0.01 M phosphate buffer at a flow rate of 1 ml/min

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However, ex vivo this may not be the case, perhaps because of limiting cysteine levels and this, potentially, may be the reason we observe increased pro-inflammatory cytokines being generated ex vivo from the blood of smokers

Of the population 50 years of age, 44% of overweight men and 48% of obese men are former smokers, whereas 27% of overweight women and 27% of obese women are former smokers