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With Davidoff Freeze we have not only created a cigarette that delivers an intense menthol taste, but also selected a filter which leaves the smoker with an exciting lip-tingling sensation. Davidoff Freeze features a menthol-impregnated inner pack, said to add to the overall smoking experience

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5 h after transfection, opti-MEM was replaced with fresh EGM-2 medium with 10% FBS