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A total of 50 g protein per sample was loaded with a volume of 5 L, separated by SDS-PAGE, and transferred onto methanol-activated PVDF membranes (IPVH00010, Millipore) at a constant current of 300 mA for 40 min

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More importantly, sulforaphane rescued TH protein levels, to over 50% of vehicle control group levels, in both lesioned brain regions (Fig