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Generation of knockout, knockdown and cDNA overexpression cell lines sgRNAs (oligonucleotide sequences are indicated below) were cloned into lentiCRISPR-v2 (Addgene) (for GCLC )or into lentiCRISPR-v1 (Addgene) (for SLC25A39 ) linearized with BsmBI by T4 ligase (NEB)

The Dual Effect of Abnormal Serum Uric Acid on Intervertebral Disc Degeneration

Bullmastiffs are big dogs that originated as watchdogs for estate owners in the 1800s to watch over game and keep their properties safe from intruders

Khanyi Mbau is no stranger to talk about her lightened complexion and she certainly ain't shy to admit that she's an advocate of using products to help her look

doi: 10.1038/s41598-022-05667-0 287 ValkoM.LeibfritzD.MoncolJ.CroninM