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These modifications drastically reduce binding affinity to IGF binding proteins (IGFBPs), resulting in a significantly extended half-life and enhanced biological activity compared to native IGF-1

The protocol includes construction and culture of the bacterial chassis strain, cell-free lysate production, assembly of freeze-dried reactions, cell-free glycoprotein synthesis, and glycoprotein characterization, all of which can be completed in one week or less

PMC 3155927

The substrate of the OGDHC reaction, 2-oxoglutarate, is a precursor for glutamate biosynthesis from glucose

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