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LC-MS/MS was performed using an Evosep LC (Evosep Biosystems) connected to a Q Exactive HF (Thermo Fisher Scientific)

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2.3 Study protocol The participants were treated with semaglutide, 1.0 mg/week in addition to their ongoing treatment of metformin at a dosage of 2000 mg/day and lifestyle interventions for 16 weeks

13.3 Gradual Dose Escalation to Minimize GI Effects Incremental titration mitigates gastrointestinal intolerance

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