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Divide the total amount of peptide in the vial by the total mL of diluent to get concentration, then calculate how much volume is needed for your dose

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His-tagged proteins were step eluted in resuspension buffer + 500 mM imidazole and injected onto a Superdex 200 26/60 gel filtration column equilibrated to 20 mM HEPES and 0.15 M NaCl, pH 7.5

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