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Blinding was assessed by asking participants which order of placebo and naltrexone pretreatments they believed they received after the final dosing session at the day 1 follow-up, with Dont know a valid option

Means were compared to the control treatment (RPMI)

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Topical peptides function as signaling molecules that communicate with fibroblasts and keratinocytes, stimulating: Collagen synthesis (types I, III, and IV) Elastin production Barrier repair and hydration retention Melanogenesis regulation (relevant to hyperpigmentation research) Key peptide classes studied in dermatology include: Peptide Class Example Primary Function Signal Peptides Palmitoyl Pentapeptide-4 (Matrixyl) Stimulates collagen and elastin production