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Paraffin sections with formalin or PLP fixation were used for immunohistochemistry for acrolein (acrolein-lysine adduct), perilipin 2, phosphorylated-histone H2AX (-H2AX), and high mobility group box protein 1 (HMGB1) as listed in Table 1

Measurement is another constraint: while lactylation can be detected by mass spectrometry and tissue assays, broadly accepted, stereochemically aware workflows and clinically validated biomarkers are still lacking

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[DOI] [PMC free article] [PubMed] [Google Scholar] 49.Staresinic M., Petrovic I., Novinscak T., Jukic I., Pevec D., Suknaic S., Kokic N., Batelja L., Brcic L., Boban-Blagaic A., et al