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General cell culture and imaging The HeLa cell line used in this study was purchased from American Type Culture Collection (ATCC) and grown in DMEM (Gibco, 11965) media supplemented with 10% FBS and 1% 1003 Pen Strep (Gibco)

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All data are expressed as the mean SD from at least three biological replicates, and comparisons between the two groups were performed with a nonparametric method (MannWhitney U test)