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Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature

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At 2.5 mg/mL, that same dose requires just 96 units, fitting comfortably in a 1 mL syringe