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Data are mean SEM, n = 3.(E) Biotin pull-down assay showing SIM1-Me is competitive with BRD-VHL-biotin for binding with CD36 in membrane fractions from LNCaP cells under indicated conditions.(F) Representative sensograms from surface plasmon resonance assays showing the binding affinity of SIM1-Me to an hCD36-coated Series S sensor chip CM5 from Cytiva