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Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

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- Angel's Liquid Glutathione Ever Collagen lm chm lo ha, ngn chn hnh thnh np nhn, ko di tui xun, nui dng ln da sn chc, n hi t nhin

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Glutamate may be converted to KG to maintain TCA cycle function and subsequently enhance the production of lipid ROS to induce ferroptosis [16,68]