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Mitochondrion is the primary cell organelle involved in ROS production

The H 2 R protein is coupled to a G s -type G-protein

For each analyte and the internal standard, the peak corresponding to the [M+H] + ion at 5ppm was integrated in Tracefinder 4.1 (Thermo Scientific)

These approaches reveal CRCs distinct metabolic signatures, enabling precise disease stratification and management

Importantly, Bcl-2 mRNA expression did not show a significant decrease in the IR group compared to the SHAM group, suggesting that anti-apoptotic signaling at the transcriptional level may not be markedly suppressed in this experimental model