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Mathematical model for glutathione dynamics in the retina

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Briefly, a RT-PCR-nested amplification was performed, and purified PCR products were quantified using the PicoGreen assay (Invitrogen, Carlsbad, CA, USA), and quality analysed using the BioAnalyzer DNA 1000 LabChip (Agilent, Santa Clara, CA, USA) prior to sequencing

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Therefore, alterations in GM can impact bile acid metabolism by engaging receptors or directly affecting bile acid synthesis, leading to disorders in bile acid metabolism ( Figure 3 )